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Relying on advanced sequencing systems and cutting-edge technologies, Creative Proteomics provides ChIP-sequencing and analysis (ChIP-Seq) services to customers around the world, aiming to help you find the DNA sequence segments that specifically bind to target proteins in the whole genome, or to study transcription factor binding sites or histone-specific modification sites.



By combining chromatin immunoprecipitation (ChIP) detection and sequencing technologies, ChIP-sequencing (ChIP-Seq) has become a powerful method to identify the DNA-binding sites of transcription factors and other protein factors on a genome-wide scale. Following the ChIP procedure, the DNA-binding proteins were immunoprecipitated by specific antibodies. The bound DNA is simultaneously precipitated, purified and sequenced. The application of next-generation sequencing (NGS) to ChIP has revealed several clues to gene regulatory events that play a role in various diseases and biological pathways, such as development and cancer progression. ChIP-Seq enables comprehensive detection of protein-nucleic acid interactions on a genome-wide scale.

Advantages of ChIP-sequencing and analysis:

  • It captures transcription factor or histone modified DNA targets throughout the genome of any organism.
  • It determines the binding sites of transcription factors.
  • It combines RNA sequencing and methylation analysis to reveal gene regulatory networks.
  • It is compatible with different starting amounts of DNA samples.

Applications of ChIP-sequencing and analysis:

  • Determine the type of histone modification at a particular position on the DNA strand.
  • Precisely locate the binding sites of RNA polymerase II and other trans-factors on the genome.
  • Study on the relationship between histone covalent modification and gene expression.
  • Transcription factor binding site and function study.

Advantages of Our ChIP-sequencing and Analysis Services

  • Strict quality control system: Creative Proteomics conducts strict quality control on all aspects of the ChIP-Seq experiment to minimize experimental risks and ensure the reliability of the results.
  • Leading technology: We have accumulated many years of IP experiment experience, and now have a mature IP experiment system, which is fast and stable.
  • Powerful sample handling: Flexibility to handle rare species and complex samples with years of experience.
  • Professional bioinformatics analysis: Our strong bioinformatics team can meet the needs of customers for in-depth data analysis.

Service Workflow

For ChIP-sequencing of experimental samples, we followed the basic workflow shown below. At the same time, Creative Proteomics has developed a variety of novel sample preparation techniques, and we will adjust the experimental methods and optimize the experimental conditions according to the different properties of the samples.

Service Workflow of ChIP-Seq -Creative Proteomics

Sample Requirements

  • ChIPed DNA ≥ 10 ng, concentration ≥ 1 ng/μL, OD260/280 = 1.8-2.0, OD260/230 ≥ 1.5
  • Fixed cells ≥ 2×107
  • Animal tissue ≥ 200mg, plant tissue ≥ 1.5 g

Bioinformatics Analysis

Type Analyze content
Standard bioinformatics analysis Alignment with reference genome
Reads distribution analysis on the genome
Sample relationship analysis (PCA analysis, sample correlation analysis)
Genome peak analysis
GO/KEGG enrichment analysis of genes associated with peak
Motif analysis of enrichment intervals
Advanced bioinformatics analysis

Differential peak analysis
Annotation Analysis of differential peaks
Distribution analysis of differential peaks on the genome
Distribution Analysis of differential peaks on gene functional elements
Distribution analysis of reads in differential peak regions

Differential peak-associated genes analysis
GO / KEGG annotation / clustering / enrichment analysis of differential peak-associated genes
Motif predictive analysis of differential peak

Personalized bioinformatics analysis Please discuss with our technical engineers to complete your personalized design analysis, such as multi-omics integrated analysis.

If you are interested in ChIP-Seq, or you want to open up new research, you need to find a professional ChIP-sequencing and analysis company for cooperation. Don't hesitate to get in touch with us today, Creative Proteomics can be trusted.

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