Overview

By combining chromatin immunoprecipitation (ChIP) detection and sequencing technologies, ChIP-sequencing (ChIP-Seq) has become a powerful method to identify the DNA-binding sites of transcription factors and other protein factors on a genome-wide scale. Following the ChIP procedure, the DNA-binding proteins were immunoprecipitated by specific antibodies. The bound DNA is simultaneously precipitated, purified and sequenced. The application of next-generation sequencing (NGS) to ChIP has revealed several clues to gene regulatory events that play a role in various diseases and biological pathways, such as development and cancer progression. ChIP-Seq enables comprehensive detection of protein-nucleic acid interactions on a genome-wide scale.

Advantages of ChIP-sequencing and analysis:

• It captures transcription factor or histone modified DNA targets throughout the genome of any organism.
• It determines the binding sites of transcription factors.
• It combines RNA sequencing and methylation analysis to reveal gene regulatory networks.
• It is compatible with different starting amounts of DNA samples.

Applications of ChIP-sequencing and analysis:

• Determine the type of histone modification at a particular position on the DNA strand.
• Precisely locate the binding sites of RNA polymerase II and other trans-factors on the genome.
• Study on the relationship between histone covalent modification and gene expression.
• Transcription factor binding site and function study.

Advantages of Our ChIP-sequencing and Analysis Services

• Strict quality control system: Creative Proteomics conducts strict quality control on all aspects of the ChIP-Seq experiment to minimize experimental risks and ensure the reliability of the results.
• Leading technology: We have accumulated many years of IP experiment experience, and now have a mature IP experiment system, which is fast and stable.
• Powerful sample handling: Flexibility to handle rare species and complex samples with years of experience.
• Professional bioinformatics analysis: Our strong bioinformatics team can meet the needs of customers for in-depth data analysis.

Service Workflow

For ChIP-sequencing of experimental samples, we followed the basic workflow shown below. At the same time, Creative Proteomics has developed a variety of novel sample preparation techniques, and we will adjust the experimental methods and optimize the experimental conditions according to the different properties of the samples.

Sample Requirements

• ChIPed DNA ≥ 10 ng, concentration ≥ 1 ng/μL, OD260/280 = 1.8-2.0, OD260/230 ≥ 1.5
• Fixed cells ≥ 2×10⁷
• Animal tissue ≥ 200mg, plant tissue ≥ 1.5 g

Bioinformatics Analysis

If you are interested in ChIP-Seq, or you want to open up new research, you need to find a professional ChIP-sequencing and analysis company for cooperation. Don't hesitate to get in touch with us today, Creative Proteomics can be trusted.