Overview

Microbial diversity sequencing, also known as amplicon sequencing, is the sequencing of PCR products or captured fragments of a specific length and then analyzing the variation in the sequence. It mainly includes 16S rDNA sequencing, 18S rDNA sequencing, ITS sequencing and target region amplicon sequencing. Firstly, by extracting the DNA of the sample, select appropriate universal primers to amplify the target region of 16S/18S/ITS/specific gene; secondly, the second-generation high-throughput sequencing platform is used to determine the sequence variation and abundance of the target region; finally, the biological information academic analysis. Reveal the differences between species in bacterial, fungal, and archaeal taxonomy of samples to study differences in microbial diversity and community composition.

Technical advantages of microbial diversity sequencing

• No need for purification culture: High-throughput sequencing technology can detect microorganisms in unculturable samples.
• "Species" can be identified: The identification of bacterial diversity is the first to be refined to "species", and the classification is more clear.
• Rich database: Based on the latest software, self-built database and self-developed annotation tools, the identified strains are continuously updated.
• Low cost: Compared with traditional colony identification, the analysis throughput is higher and the detection cost is lower.

Applications of microbial diversity sequencing

• Medical field: Research on the relationship between common diseases and human microorganisms.
• Animal field: Gut, rumen (such as methanogens) and animal health/nutrient digestion research, etc.
• Agricultural field: Interaction between rhizosphere microorganisms and plants, agricultural cultivation/fertilization treatment and soil microbial community research, etc.
• Environmental field: Haze treatment, sewage treatment, petroleum degradation, acid mineral water treatment and marine environment research, etc.
• Special extreme environments: Microbial taxa studies under extreme environmental conditions.

Microbial Diversity Sequencing Services We Offer

• 16S amplicon sequencing
• 18S amplicon sequencing
• ITS amplicon sequencing
• Functional gene amplicon sequencing

Advantages of Our Microbial Diversity Sequencing Services

• Creative Proteomics provides flexible and changeable research solutions according to the different research needs of customers.
• We have several platforms for you to choose from, including ultra-long-reads platforms and short-reads platforms.
• A variety of optional primers are available for different types of microorganisms.
• We can integrate microbial diversity sequencing and metabolomic / proteomic data for multi-omics integrated analysis to deepen research in all aspects.
• A variety of personalized analysis solutions to meet research needs in an all-round way and help scientific research succeed.
• Professional and mature sample preparation, library construction and data analysis processes.

Service Workflow

For microbial diversity sequencing and analysis, we followed the basic workflow shown below. At the same time, Creative Proteomics has developed a variety of new sample preparation technologies, and we will adjust the experimental methods and optimize the experimental conditions according to the different properties of the samples.

Sample Requirements

• Soil ≥ 10 g
• Feces = 3-5 g
• Blood ≥ 10 mL
• Sludge / sediment = 5-10 g
• Total DNA ≥ 1 μg, concentration ≥ 20 ng/μL

Bioinformatics Analysis

Our analysis content is constantly being updated, so if you would like to know more, please contact our technical engineers.

Creative Proteomics provides detailed microbial diversity sequencing and analysis services, please contact us for more information. We deserve your trust.