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Microbial Diversity Sequencing

Relying on advanced sequencing systems and cutting-edge technologies, Creative Proteomics provides global customers with microbial diversity sequencing and analysis services, including 16S rDNA sequencing, 18S rDNA sequencing, ITS sequencing and target region amplicon sequencing. The dominant species, rare species and some unknown species in the sample are detected to obtain the composition of the microbial community in the sample and the relative abundance between them. Revealing the differences between species in the classification of bacteria, fungi and archaea in environmental samples has an important guiding role in the study of microbial composition in the ocean, soil, intestinal feces and other environments.


Microbial Diversity Sequencing

Microbial diversity sequencing, also known as amplicon sequencing, is the sequencing of PCR products or captured fragments of a specific length and then analyzing the variation in the sequence. It mainly includes 16S rDNA sequencing, 18S rDNA sequencing, ITS sequencing and target region amplicon sequencing. Firstly, by extracting the DNA of the sample, select appropriate universal primers to amplify the target region of 16S/18S/ITS/specific gene; secondly, the second-generation high-throughput sequencing platform is used to determine the sequence variation and abundance of the target region; finally, the biological information academic analysis. Reveal the differences between species in bacterial, fungal, and archaeal taxonomy of samples to study differences in microbial diversity and community composition.

Type Research object
16S rDNA/rRNA Bacteria or archaea
18S rDNA/rRNA Eukaryotes
ITS sequencing Fungus
Functional gene amplicon sequencing Sequencing PCR products of specific genes

Technical advantages of microbial diversity sequencing

  • No need for purification culture: High-throughput sequencing technology can detect microorganisms in unculturable samples.
  • "Species" can be identified: The identification of bacterial diversity is the first to be refined to "species", and the classification is more clear.
  • Rich database: Based on the latest software, self-built database and self-developed annotation tools, the identified strains are continuously updated.
  • Low cost: Compared with traditional colony identification, the analysis throughput is higher and the detection cost is lower.

Applications of microbial diversity sequencing

  • Medical field: Research on the relationship between common diseases and human microorganisms.
  • Animal field: Gut, rumen (such as methanogens) and animal health/nutrient digestion research, etc.
  • Agricultural field: Interaction between rhizosphere microorganisms and plants, agricultural cultivation/fertilization treatment and soil microbial community research, etc.
  • Environmental field: Haze treatment, sewage treatment, petroleum degradation, acid mineral water treatment and marine environment research, etc.
  • Special extreme environments: Microbial taxa studies under extreme environmental conditions.

Microbial Diversity Sequencing Services We Offer

  • 16S amplicon sequencing
  • 18S amplicon sequencing
  • ITS amplicon sequencing
  • Functional gene amplicon sequencing

Advantages of Our Microbial Diversity Sequencing Services

  • Creative Proteomics provides flexible and changeable research solutions according to the different research needs of customers.
  • We have several platforms for you to choose from, including ultra-long-reads platforms and short-reads platforms.
  • A variety of optional primers are available for different types of microorganisms.
  • We can integrate microbial diversity sequencing and metabolomic / proteomic data for multi-omics integrated analysis to deepen research in all aspects.
  • A variety of personalized analysis solutions to meet research needs in an all-round way and help scientific research succeed.
  • Professional and mature sample preparation, library construction and data analysis processes.

Service Workflow

For microbial diversity sequencing and analysis, we followed the basic workflow shown below. At the same time, Creative Proteomics has developed a variety of new sample preparation technologies, and we will adjust the experimental methods and optimize the experimental conditions according to the different properties of the samples.

Service Workflow of Microbial Diversity Sequencing -Creative Proteomics

Sample Requirements

  • Soil ≥ 10 g
  • Feces = 3-5 g
  • Blood ≥ 10 mL
  • Sludge / sediment = 5-10 g
  • Total DNA ≥ 1 μg, concentration ≥ 20 ng/μL

Bioinformatics Analysis

Our analysis content is constantly being updated, so if you would like to know more, please contact our technical engineers.

Analysis type Analyze content
Basic analysis QC / filtering / dechimerization
OTU clustering
OTU abundance
Species annotation
Alpha diversity analysis Dilution curve
Diversity index
Diversity index box plot
Shannon curve
Rank abundance curve
Beta diversity analysis Distance analysis between groups
Anosim / adonis
Multi-sample clustering tree
Unifrac phylogenetic tree
Species composition analysis Venn diagram
Community structure composition map
Core microbial analysis
Species composition heatmap
Dominant species composition map
Multilevel species composition chart
graphlan species composition tree
Difference analysis Anova variance analysis
Wilcoxon analysis
Kruskal wallis test
Metastat analysis
STAMP difference analysis
Difference comparison histogram of key species
Correlation analysis RDA / CCA analysis
Correlation heatmap
Correlation network diagram
Mantel test analysis
network composition analysis
Procrustes analysis
16s function prediction Picrust function prediction
Tax4Fun function prediction
Functional distribution map
Differential functional analysis
Species, functions, and physicochemical binding assays
Functional comparative analysis

Creative Proteomics provides detailed microbial diversity sequencing and analysis services, please contact us for more information. We deserve your trust.

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