Why Do RNA-seq and ATAC-seq Integration Analysis?

Assay for transposase-accessible chromatin sequencing (ATAC-seq) can achieve two main goals: (1) Which genes are involved in the regions where genome opening changes occur between specific grouped samples. That is, which are the target genes regulated by genome opening changes. (2) which transcription factor binding sites are enriched in the regions of genome opening changes. That is, what are the upstream regulatory transcription factors of the genomic open changes. As the genome openness changes, the expression changes of the upstream regulatory factors of the source, whose effects in turn lead to the expression changes of the downstream target genes. Therefore, RNA-seq combined with ATAC-seq is the best combination to study how genomic openness regulates gene transcriptional changes.

Our Integrative Analysis Services of RNA-seq and ATAC-seq

Creative Proteomics provides you with RNA sequencing and ATAC sequencing services. In addition, we provide integrative analysis services for RNA-seq and ATAC-seq, seamlessly connecting your experiments to provide powerful insights into the evidence of complex life activity mechanisms.

• mRNA Sequencing
• ATAC-seq
• scATAC-seq

Bioinformatics analysis Process and Content

For the integration analysis of RNA-seq and ATAC-seq, we conducted the analysis from 4 perspectives:

1. From the sample level, the correlation between RNA-seq and ATAC-seq across samples was assessed.
2. Within treatment groups, the relationship between genomic openness and gene expression was analyzed.
3. Between treatment groups, analyze the effect of gene proximal/distal genomic openness on gene expression, and expect that from this, key pathways / genes that are differentially expressed by changes in openness can be screened.
4. The core TFs were targeted and regulatory networks were mapped using RNA-seq data and ATAC-seq data.

Delivery Content

Creative Proteomics will provide you with a detailed report, including:

• Raw sequencing data
• Related analysis software and parameters used
• Graphs obtained from the integrated analysis

Service Process

Why Choose Us?

• One-stop service. Creative Proteomics provides a complete set of services from sample preparation, library preparation, high-throughput sequencing, bioinformatics analysis, to comprehensive multi-omics analysis.
• Relying on a strong bioinformatics team and a variety of analysis software, Creative Proteomics provides high-end and personalized bioinformatics data analysis services to meet customers' various data analysis needs.

Creative Proteomics relies on advanced platforms and cutting-edge technologies to provide omics services to customers around the world, and provide accurate and detailed analysis reports. In addition, we also provide multi-omics integrated analysis services. If you want to know more, please contact us, Creative Proteomics can be trusted.