Overview

Although lncRNAs have been discovered in recent years with the widespread use of gene microarrays and second-generation high-throughput sequencing technologies, the exact function of such transcripts is unknown. The lncRNA microarrays currently available in the market usually design lncRNA and mRNA together, and RNASeq data also contain lncRNA and mRNA sequence information, so lncRNA can be functionally annotated by analyzing the correlation between lncRNA and mRNA expression.

Our Integrative Analysis Services of mRNA and IncRNA

Creative Proteomics provides you with one-stop mRNA and IncRNA sequencing services. In addition, we provide comprehensive analysis services for mRNA and IncRNA for sequencing data or microarray data, seamlessly connecting your experiments to provide powerful insights into the evidence of complex life activity mechanisms.

• mRNA Sequencing
• lncRNA Sequencing
• ChIP Data

Bioinformatics Analysis

1. Calculate the lncRNA-mRNA expression correlation, screen the lncRNA-mRNA relationship pairs according to the set domain values, and construct the lncRNA-mRNA co-expression network.
2. Get the potential target genes of lncRNA based on the lncRNA-mRNA expression correlation and the relationship between lncRNA and mRNA position in the genome. Functional annotation and functional enrichment analysis of differentially expressed lncRNA target genes were performed, such as Barplot of GO enrichment and Heatmap of differential lncRNAs.
3. To study the topological properties of the co-expression network of lncRNAs and mRNAs, and to screen the network topologically important lncRNAs based on the degree, which are most likely to be lncRNAs related to the study context. thus the local co-expression sub-networks of important lncRNAs and mRNAs can be constructed.
4. Customers provide a set of genes related to the study context, and based on the expression correlation the lncRNAs related to this set of genes can be identified, thus constructing a co-expression network of interest. The constructed co-expression network can further find the hub lncRNAs of interest.

Deep mining analysis of lncRNAs

• Target gene prediction of differential lncRNAs
• Gene Ontology analysis of target genes
• Pathway analysis of target genes
• Expression mechanism of lncRNAs and regulatory genes
• Transcription factor binding site prediction
• Gene association analysis
• Signaling pathway regulatory network construction
• Functional analysis of lncRNAs

Delivery Content

Creative Proteomics will provide you with a detailed report, including:

• Raw sequencing data
• Related analysis software and parameters used
• Graphs obtained from the integrated analysis

Service Process

Why Choose Us?

• Many years of project analysis experience, rich project analysis cases.
• Professional bioinformatics analysis. Creative Proteomics has a strong bioinformatics team, which can meet various in-depth data analysis needs of customers, and can also provide integrated analysis services with other omics.
• One-stop service. Customers only need to provide samples, and Creative Proteomics will complete the full-service process from sample preparation, library preparation, high-throughput sequencing / Mass detection to bioinformatics analysis, as well as integrated analysis of multi-omics.
• Tailored and personalized analysis content is available for special projects.

Creative Proteomics relies on advanced platforms and cutting-edge technologies to provide omics services to customers around the world, and provide accurate and detailed analysis reports. In addition, we also provide multi-omics integrated analysis services. If you want to know more, please contact us, Creative Proteomics can be trusted.