Whole Genome Sequencing (WGS)
Relying on advanced sequencing systems and cutting-edge technologies, Creative Proteomics provides global customers with whole genome sequencing (WGS) and analysis services, enabling comprehensive and accurate analysis of the entire genome, so as to decipher the information contained therein and reveal the complexity and diversity of the genome.
Overview
Whole genome sequencing (WGS) can obtain the entire coding (exon) and non-coding regions of the genome, which is the data of each gene and all chromosomes in the genomic DNA, that is, a complete and comprehensive map of genome composition. It allows extensive analysis of all genes.
Technical advantages of whole genome sequencing (WGS):
- High-frequency, low-frequency, and even rare point mutations and structural variation information associated with phenotypes can be detected at the genome-wide level.
- For individual samples, comprehensive genomic mutational profile information is available.
- For population samples, the evolutionary history, environmental adaptation, natural selection and trait localization of species can be further studied.
- It has high accuracy, good reproducibility and precise positioning, and has been widely used in genomic research of diseases and cancers.
Application of whole genome sequencing (WGS):
- Genome-wide mapping of multiple traits
- Biological basic research of target traits
- Discover common/rare mutations
- Discovering a link between DNA mutations and a phenotype
- Disease risk screening
- Pathogenesis, metastasis / recurrence mechanisms of cancer and tumors
- Genetic disease direction
- Pharmacogenomics
Advantages of Our Whole Genome Sequencing (WGS) Services
- High data quality, short project cycle and high cost performance.
- Tailored and personalized analysis content is available for special projects.
- With years of experience in molecular biology services, we can design flexible solutions for a variety of complex samples (gDNA, cells, blood, fresh tissue, frozen tissue, paraffin section FFPE, prepared libraries, etc.).
Service Workflow
For whole genome sequencing (WGS) of experimental samples, we followed the basic workflow shown below. At the same time, Creative Proteomics has developed a variety of new sample preparation technologies, and we will adjust the experimental methods and optimize the experimental conditions according to the different properties of the samples.
Sample Requirements
- Cell/tissue samples: Tissue samples > 1 mg, cell samples > 1x107
- Body fluid sample: Peripheral blood ≥ 3mL
- DNA ≥ 5 μg, concentration ≥ 300 ng/μL
- DNA from fresh or frozen samples ≥ 0.2 μg, DNA of FFPE samples ≥ 1μg
Bioinformatics Analysis
| Analysis type |
Analyze content |
| Standard bioinformatics analysis |
Sequencing data quality control analysis
Alignment analysis with reference genome sequences
Single nucleotide polymorphism (SNP) site detection
Gene copy number variation (CNV) analysis
Structural variant site detection |
| Advanced bioinformatics analysis |
QTL mapping for traits (eukaryotes)
Population evolution analysis
Functional gene mining |
| Personalized bioinformatics analysis |
Customized analysis according to customer needs. |
Creative Proteomics provides detailed whole genome sequencing (WGS) and analysis services, please contact us for more information. We deserve your trust.
Related Services
For Research Use Only.
