Integrative Analysis of RNC-seq and RNA-seq
Based on the advanced technology platform, Creative Proteomics provides translationomics sequencing (RNC-seq) and transcriptomics sequencing (mRNA-seq) services and integrated analysis of RNCseq-RNAseq.
Why Do RNC-seq and RNA-seq Integration Analysis?
Much of the purpose of studying the translatome is to investigate the portion of the sample genes that are being translated to synthesize proteins and the translational changes in the genes under different experimental treatment conditions. Only in combination with the transcriptome can post-transcriptional regulation be studied in more depth. The Ribosome Nascent-chain Complex (RNC) is the most important unit in the translation process. the ratio of RNC-mRNA to total mRNA is the translation ratio (TR). The TR is related to the phenotype of the cell and is also regulated by UTR, miRNA, etc. Comparing TR-altered genes in different samples can study the molecular mechanisms of disease development, drug action, development, etc. It also provides a powerful tool to elucidate the molecular mechanisms of ncRNAs such as miRNAs.
Main research strategies
| Probing the mechanism of a gene's effect on a disease |
(1) Screening genes that are in high translational efficiency among genes being translated and exploring their global functional impact on disease to guide subsequent experiments.
(2) Exploring the impact of unconventional translation phenomena on disease. |
| Deep transcriptome sequencing analysis |
(1) Identify the genes being translated in differentially expressed genes and the efficiency of translation.
(2) Narrowing the range of differentially expressed genes in the transcriptome, screening for genes that are being translated, or comparing their translation efficiencies.
(3) Expand the range of differentially expressed genes in the transcriptome, then additional differentially expressed / translated genes can be identified through transcriptomic analysis. |
| Explain the discordance between transcriptomic and proteomic results |
If transcriptomics shows differential or significant differences in gene expression, while proteomic validation reveals no differences or insignificant differences, possibly because the genes are transcribed but not translated or translated inefficiently. |
| Probe whether a certain lncRNA is translated into a short peptide or protein. |
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Our Integrative Analysis Services of RNC-seq and RNA-seq
Creative Proteomics provides you with translationomics sequencing (RNC-seq) and transcriptomics sequencing (RNA-seq) services. In addition, we provide integrative analysis services for RNC-seq and RNA-seq, seamlessly connecting your experiments to provide powerful insights into the evidence of complex life activity mechanisms.
Bioinformatics Analysis
- Data quality control
- Map sequence reads to reference genome
- Gene expression level analysis
- Differential translation efficiency gene (DTEG) analysis: screening, clustering analysis, GO/KEGG enrichment analysis
- Nine-quadrant diagram (comparative analysis of transcriptional and translational differences in genes)
- Translation ratio (TR) analysis (transcriptomic data required)
- Correlation scatter plot of translation efficiency and transcriptome (negative Pearson correlation coefficient indicates higher gene transcription levels and lower translation efficiency)
- Translational efficiency (TE) abundance distribution density plot or box plot (compare the overall level of translational efficiency in different treatment groups)
Delivery Content
Creative Proteomics will provide you with a detailed report, including:
- Raw sequencing data
- Related analysis software and parameters used
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Graphs obtained from the integrated analysis
Service Process
Why Choose Us?
- Many years of project analysis experience, rich project analysis cases.
- Professional bioinformatics analysis. Creative Proteomics has a strong bioinformatics team, which can meet various in-depth data analysis needs of customers, and can also provide integrated analysis services with other omics.
- One-stop service. Customers only need to provide samples, and Creative Proteomics will complete the full-service process from sample preparation, library preparation, high-throughput sequencing / Mass detection to bioinformatics analysis, as well as integrated analysis of multi-omics.
- Tailored and personalized analysis content is available for special projects.
Creative Proteomics relies on advanced platforms and cutting-edge technologies to provide omics services to customers around the world, and provide accurate and detailed analysis reports. In addition, we also provide multi-omics integrated analysis services. If you want to know more, please contact us, Creative Proteomics can be trusted.
For Research Use Only.
