Microbial Re-sequencing
With advanced sequencing systems and cutting-edge technology, Creative Proteomics provides microbial resequencing and analysis services to customers worldwide, including bacterial resequencing and fungal resequencing. We help you to obtain a series of variation information (SNP, InDel, SV, etc.) of the target microbial genome to the reference genome. As a result, you can try to predict and resolve the trait differences between genomes, and thus complete the study of target microorganisms.
Overview
Microorganisms are a large group of organisms including bacteria, viruses, fungi, and some small protozoa, microscopic algae, etc. Although they are tiny in size, they are closely related to nature. Microbial genome resequencing mainly refers to the resequencing of the genome of bacteria or fungi. The principle is to perform high-throughput sequencing based on small fragment libraries, and then compare the sequencing data with the close reference genome to obtain the SNP, InDel, SV and other variation information of the strain and the reference genome. Microbial resequencing can rapidly resolve the variation information among strains for studies on species evolution, population characteristics and selection pressure.
Technical advantages of microbial re-sequencing
- Comprehensive analysis: Systematic and comprehensive detection of variant information.
- Wide range of studies: This allows studies of species evolution, population characteristics, selection pressure, etc.
Application of microbial re-sequencing
- Research on environmental microorganisms, marine microorganisms, soil microorganisms, industrial microorganisms, and organism microorganisms, etc.
- Research on pathogenic bacteria, probiotic bacteria, edible bacteria, medicinal strains, industrial strains, etc.
Advantages of Our Microbial Re-sequencing Services
- From material selection, library construction and sequencing to data analysis, each step is scientifically and meticulously designed to guarantee high quality research results.
- We have rich project experience, professional analysis team, and high quality project service to help scientific research.
Service Workflow
For microbial re-sequencing and analysis, we followed the basic workflow shown below. At the same time, Creative Proteomics has developed a variety of new sample preparation technologies, and we will adjust the experimental methods and optimize the experimental conditions according to the different properties of the samples.
Sample Requirements
- Bacterial DNA ≥ 3 μg, concentration ≥ 20 ng/μL. Requirements for size fragment libraries vary.
- Bacterial powder > 1 g, bacterial fluid > 5 mL.
- Fungal DNA ≥ 3 μg, concentration ≥ 20 ng/μL.
- Fungal tissue >1 g.
- Electrophoresis requirements: clear main band, no degradation.
- Try to provide larger amounts, the yield of DNA extraction varies among species.
Bioinformatics Analysis
Our analysis content is constantly being updated, so if you would like to know more, please contact our technical engineers.
| Analysis type |
Analysis content |
Problem solving |
| Standard bioinformatics analysis & Advanced bioinformatics analysis |
Data quality control and statistics
Genome assembly
Genome component analysis
- Coding gene prediction
- Repeat sequences
- tRNA / rRNA lookup
- Pre-phage
Comparative analysis
- SNP statistics and annotation
- Indel statistics and annotation
- Species evolution analysis
- Shared / specific gene analysis
- Gene family analysis
- Genome-wide covariance analysis
|
Mutation detection: accurate localization of variant loci.
Evolutionary analysis: construct phylogenetic trees to determine the evolutionary relationships among strains.
Selection pressure analysis: reveal key genes associated with the environment.
Functional enrichment of variant genes: search for variant-rich functional classifications. |
| Personalised bioinformatics analysis |
Personalised bioinformatics analysis can be negotiated with the client's needs. |
Creative Proteomics provides detailed microbial re-sequencing and analysis services, please contact us for more information. We deserve your trust.
Related Services
For Research Use Only.
