Lipidomics
Relying on advanced sequencing systems and cutting-edge technologies, Creative Proteomics provides global customers with lipidomics services. We provide one-stop service for lipid extraction, isolation, detection, identification and bioinformatics analysis to help you conduct refined lipid studies.
Overview
Lipids are a large group of chemically and structurally non-homogeneous compounds found in nature that are highly soluble in organic solvents. They include mainly fatty acids and their naturally occurring derivatives, as well as compounds related to their biosynthesis and function. According to the latest LIPID MAPS classification, lipids are classified into eight major groups in terms of chemical structure and biosynthesis, namely: fatty acyls, glycerolipids, glycerol-phospholipids, sphingolipids, sterollipids, prenolipids, saccharo-lipids, polyketides.
Lipidomics is a branch of metabolomics and is generally considered to be the study of the properties of all lipid molecules in an organism and their role in protein expression and gene regulation.
There are many established methods for lipid analysis, mainly TLC, GC-MS, ESI-MS, MALDITOF-MS, NMR, etc. The more advanced ESI-MS method is capable of qualitative and quantitative analysis of the molecular species of lipids and has become a powerful tool for lipid analysis.
Applications of lipidomics
- Agriculture: animal / plant growth and development, plant resistance research
- Medical field: animal / plant physiology and pathology, biomarker discovery, drug mechanism of action research
Lipidomics Services We Offer
- Relative quantification of the lipidome
- Absolute quantification of the lipidome
- High throughput targeted lipidome
- Targeted lipidome
Advantages of Our Lipidomics Services
- Platform advantages: orbitrap mass analyzer and ultra-high resolution mass spectrometry for high quality data.
- High quality methods: optimised chromatographic methods with high peak volumes to ensure optimal results.
- Strict quality control: multiple quality controls to ensure data reliability.
- Comprehensive data: detailed analytical reports, rich analytical content and visual displays are provided.
Service Workflow
For lipidomics services, we followed the basic workflow shown below. At the same time, Creative Proteomics has developed a variety of new sample preparation technologies, and we will adjust the experimental methods and optimize the experimental conditions according to the different properties of the samples.
Sample Requirements
- Cells ≥ 107
- Plasma, serum ≥ 200 μL
- Liver, kidney, carcinoma / paraneoplastic tissue, etc. ≥ 200mg
Recommended Biological Replicates
The number of biological repetitions required for each group of samples is as follows, the more repetitions the better.
- Clinical samples ≥ 30 cases / group
- Animal source ≥ 10 cases / group
- Cell samples ≥ 6 cases / group
Bioinformatics Analysis
| Lipidomics services |
Analysis content |
| Relative quantification of the lipidome |
Standard bioinformatics analysis
- Quality Control (TIC, QC, etc.)
- Profile analysis (Superclass classification)
- Variance analysis (univariate statistical analysis, multidimensional statistical analysis (PCA, PLSDA, OPLSDA, etc.))
- Functional analysis (hierarchical cluster analysis, correlation analysis, KEGG analysis)
Advanced bioinformatics analysis
- Regression analysis
- Trend analysis
|
| Absolute quantification of the lipidome |
Standard bioinformatics analysis
- Quality Control (TIC, QC, etc.)
- Profile analysis (lipid molecules classification)
- Variance analysis (univariate statistical analysis, multidimensional statistical analysis (PCA, PLSDA, OPLSDA, etc.))
- Functional analysis (hierarchical cluster analysis, correlation analysis)
Advanced bioinformatics analysis
- Regression analysis
- Trend analysis
|
| High throughput targeted lipidome |
Standard bioinformatics analysis
- Quality Control (TIC, QC, etc.)
- Profile analysis (lipid molecules classification)
- Variance analysis (univariate statistical analysis, multidimensional statistical analysis (PCA, PLSDA, OPLSDA, etc.))
- Functional analysis (hierarchical cluster analysis, correlation analysis)
Advanced bioinformatics analysis
- Regression analysis
- Trend analysis
|
| Targeted lipidome |
Standard bioinformatics analysis
- Quality Control (TIC, QC, etc.)
- Methods evaluation
- Variance analysis
Advanced bioinformatics analysis
|
Creative Proteomics provides detailed lipidomics services, please contact us for more information. We deserve your trust.
Related Services
For Research Use Only.
