Creative Proteomics provides proteome and lipidomics services based on advanced tandem mass spectrometers and software, and provides customized analysis solutions and fast and accurate analysis services. Creative Proteomics can provide you with one-stop proteomics and lipidomics integrated analysis services from experimental design, sample detection, data analysis, etc., to meet a variety of detection needs.
Why Do Integrative Analysis of Proteomics and Lipidomics?
The integrated analysis of proteomics and lipidomics is to normalize and statistically analyze the batch data from the proteome and lipidome to establish the data relationship between the two different levels of proteins and lipids. Combined with functional analysis, metabolic pathway enrichment, molecular interaction and other biological function analysis, we can further systematically and comprehensively analyze the function and regulatory mechanism of biomolecules, and achieve a comprehensive understanding of the general trend and direction of biological changes. Then, a molecular biology change mechanism model is proposed, and key metabolic pathways or genes and lipid metabolites are screened out for subsequent in-depth experimental analysis and application.
Our Integrative Analysis Services of Proteomics and Lipidomics
Creative Proteomics provides you with one-stop proteome and lipidome detection services. In addition, we offer integrative analysis services of proteomics and lipidomics to seamlessly connect your experiments upstream and downstream. To mine the correlation and mutual verification of proteome and lipidome levels; screen key proteins / lipids / metabolic pathways from massive data to provide powerful insights into the mechanism of complex life activities evidence. Then analyze the development of biological system processes, explain the complexity and integrity of biological processes.
Bioinformatics Analysis
To obtain the set of relevant proteins and lipids that have an impact on the sample grouping and to analyze their association characteristics, we performed the following analyses mainly based on the expression abundance and function of differential proteins and differential lipids: pathway integration analysis of proteomics - lipidomics, multivariate statistical integration analysis of proteomics - lipidomics, and correlation analysis of proteomics and lipidomics.
Delivery Content
Creative Proteomics will provide you with a detailed technical report, including:
- Experimental procedure (LC-MS/MS)
- Mass spectrum picture
- Raw data of lipidomics data and proteomics data
- Related analysis software and parameters used
- Graph of results of integrated analysis of lipidomics and proteomics data
Why Choose Us?
- Excellent bioinformatics analysis - our bioinformatics center brings together many high-level information analysis talents from home and abroad, and has many years of practical experience in the field of bioinformatics research and development.
- In addition to excellent bioinformatics analysis, we also have an advanced experimental platform that supports sequencing and metabolome / proteome / lipidome detection, etc., and we have many successful project experiences.
Case Study
Proteomics and lipidomics analyses reveal modulation of lipid metabolism by perfluoroalkyl substances in liver of Atlantic cod (Gadus morhua)
Journal: Aquatic Toxicology
Impact factor: 4.344
Published: 2020
Backgrounds
The aim of the present study was to investigate effects of defined mixtures of polycyclic aromatic hydrocarbons (PAHs) and perfluoroalkyl substances (PFASs), at low, environmentally relevant (1× = L), or high (20× = H) doses, on biological responses in Atlantic cod (Gadus morhua).
Materials & Methods
Atlantic cod (Gadus morhua)
Proteomics and lipidomics
| Integrative analysis of proteomics + lipidomics |
|---|
Analysis of the hepatic proteome identified that pathways related to lipid degradation were significantly affected by PFAS exposure, including upregulation of enzymes in fatty acid degradation pathways, such as fatty acid β-oxidation. The increased abundances of enzymes in lipid catabolic pathways paralleled with decreasing levels of triacylglycerols (TGs) in the H-PFAS exposure group, suggest that PFAS increase lipid catabolism in Atlantic cod. Markers of oxidative stress, including catalase and glutathione S-transferase activities were also induced by PFAS exposure. Only minor and non-significant differences between exposure groups and control were found for cyp1a and acox1 gene expressions, vitellogenin concentrations in plasma, Cyp1a protein synthesis and DNA fragmentation. In summary, our combined proteomics and lipidomics analyses indicate that PFAS may disrupt lipid homeostasis in Atlantic cod. |
Conclusions
PFAS may disrupt lipid homeostasis in Atlantic cod.
Fig.1 Scores plot and Variable Importance in Projection (VIP) scores from PLS-DA multivariate analysis of liver microsomal lipidome in cod exposed to H-PFAS. (Karina Dale, et al., 2020)
Creative Proteomics relies on advanced platforms and cutting-edge technologies to provide omics services to customers around the world, and provide accurate and detailed analysis reports. In addition, we also provide multi-omics integrated analysis services. You can discuss with our technical engineers to customize solutions or build new methods to meet your specific needs. If you have any questions, you can tell us through the inquiry form, and our technicians will communicate with you. Please don't hesitate to contact us, Creative Proteomics can be trusted.
Reference
- Karina Dale, Fekadu Yadetie, Mette Bjørge Müller, Daniela M. Pampanin, Alejandra Gilabert, Xiaokang Zhang, Zhanna Tairova, Ane Haarr, Roger Lille-Langøy, Jan Ludvig Lyche, Cinta Porte, Odd André Karlsen, Anders Goksøyr, Proteomics and lipidomics analyses reveal modulation of lipid metabolism by perfluoroalkyl substances in liver of Atlantic cod (Gadus morhua), Aquatic Toxicology, Volume 227, 2020, 105590, ISSN 0166-445X.