Integrative Analysis of 16S and Metabolomics

Why Do Integrative Analysis of 16S and Metabolomics?

16S analysis obtained the distribution characteristics of species in the sample and the difference species between groups. However, metabolites are more related to phenotype. For example, some metabolites produced by microorganisms in the human/animal gut may cause disease or treatment of the host; the metabolites of microorganisms in the fermentation process also directly affect the fermentation quality. Therefore, in order to further analyze which species through which metabolites produce intergroup differences from a large population of microorganisms and metabolites, or to study the relationship between gut microbiota and host metabolic phenotypes and how microbial communities affect metabolic function, It is necessary to carry out the association analysis of 16S and metabolites.

Our Integrative Analysis Services of 16S and Metabolomics

Creative Proteomics provides you with one-stop 16S sequencing and metabolomics testing services. In addition, we provide comprehensive analysis services for 16S and metabolomics, seamlessly connecting your experiments to provide powerful insights into the evidence of complex life activity mechanisms. In addition, combining microbiomics and metabolomics can help in-depth study of how the gut microbial community affects metabolic function and the metabolite-microbe-disease relationship.

• Microbial Diversity Sequencing (16S Sequencing)
• Targeted Metabolomics
• Untargeted Metabolomics

Bioinformatics Analysis

Based on the omics data of samples in which microbial 16S and metabolome (untargeted / targeted metabolome) correspond one-to-one, we first screened differential strains and metabolites between groups from the 16S and metabolic levels, respectively; then we performed an integrated analysis based on their expression quantity / function correlation. In this way, the correlation between specific flora and specific metabolites (groups) is revealed, and the key strains that cause changes in the metabolic state or functional metabolic small molecules of the flora are screened.

In the study of the association mechanism of microorganisms and hosts, 16S analysis obtained information such as species distribution characteristics of samples and different species between groups, while transcriptomics can reflect the expression activity of hosts. In order to further explore the specific molecular mechanism of the relationship between the health, disease, growth, and stress resistance of microorganisms and hosts (humans, animals and plants, etc.), the association analysis of species and genes / functions can also be carried out. Therefore, in order to identify which species are associated with which gene expression from the huge collection of microorganisms and genes, and the differences in grouping arise, it is necessary to perform 16S-transcriptomics association analysis.

Our Integrative Analysis Services of RNA-seq and 16S

Creative Proteomics provides you with one-stop RNA-seq and 16S sequencing services. In addition, we offer integrated analysis services of RNA-seq and 16S, seamlessly connecting your experiments to provide powerful insights into the evidence of complex life activity mechanisms.

Bioinformatics Analysis

Based on the sample data of one-to-one correspondence between microorganisms and transcriptomes, in the integrated analysis, we will use three models: (1) O2PLS model (bidirectional orthogonal projections to latent structures), which uses data on species abundance and gene expression to build a model. Through model prediction, the associated species and gene sets are obtained. (2) Correlation coefficient model (sample group ≥ 3), calculate the pearson correlation coefficient of species abundance and gene expression, and display them in heat map and network diagram. (3) CCA model (canonical correlation analysis), which analyzes the relationship between genes and species distribution in the form of dimensionality reduction (the gene name used for CCA analysis needs to be provided).

Delivery Content

Creative Proteomics will provide you with a detailed report, including:

• Related analysis software and parameters used
• Graphs obtained from the integrated analysis

Why Choose Us?

• Professional bioinformatics analysis. Creative Proteomics has a strong bioinformatics team, which can meet various in-depth data analysis needs of customers, and can also provide integrated analysis services with other omics.
• One-stop service. Customers only need to provide samples, and Creative Proteomics will complete the full-service process from sample preparation, library preparation, high-throughput sequencing / Mass detection to bioinformatics analysis, as well as integrated analysis of multi-omics.
• Tailored and personalized analysis content is available for special projects.