Overview

Microbial (fungal and bacterial) genome de novo sequencing is a technique for sequencing, de novo assembly of microbial (fungal and bacterial) genome sequences without a reference genome, and annotation of the genome based on a database, as a basis for structural and functional analysis. With the development of sequencing technologies and the reduction of sequencing costs, it has become inevitable to complete a whole genome sequence map of a microorganism (fungal and bacteria). The completion of a genome sequence map of a species will lead to a series of subsequent studies of that species.

The use of de novo sequencing to obtain genomic information of microorganisms provides a powerful research tool for exploring gene structure and function, studying the evolutionary origins of microbial populations, and provides a new starting point for potential applications in medicine, disease, agriculture and farming.

Technical advantages of microbial genome de novo sequencing

• Wide range of applications: de novo sequencing, gene annotation, comparative genomic studies, microbial evolution studies, etc.
• Time saving and cost effective.

Applications of microbial genome de novo sequencing

• Construction of engineered bacteria for food, energy and other industries
• Genetic evolutionary analysis of fungi / fungus
• Pathogenicity migration mechanisms
• Research on virulence studies, drug resistance mechanisms, molecular markers, vaccine development, etc.
• Auxiliary multi-omics analysis

Advantages of Our Microbial Genome De Novo Sequencing Services

• Powerful sequencing capabilities: We rely on multiple sequencing platforms that can combine next-generation sequencing (NGS) and long-reads sequencing strategies to provide flexible options for microbial (bacterial and fungal) genome sequencing, improving the integrity and reliability of genome sequence assembly. Suitable solutions are tailored for different fineness levels and different research contexts.
• Deep data mineable: we have complete solutions for in-depth analysis of individual variation and interpretation of population evolutionary history to address the biological questions of your interest.
• Accuracy: Create accurate reference sequences even for complex or polyploid genomes.

Service Workflow

For microbial genome de novo sequencing and analysis, we followed the basic workflow shown below. At the same time, Creative Proteomics has developed a variety of new sample preparation technologies, and we will adjust the experimental methods and optimize the experimental conditions according to the different properties of the samples.

Sample Requirements

• Fungal / bacterial samples ≥ 5 g.
• Framework map genomic DNA ≥ 2 µg, completed framework map genomic DNA ≥ 10 µg, concentration ≥ 30 ng/µL, OD260/280 = 1.8-2.0, and ensure no degradation of DNA and no contamination.      

Bioinformatics Analysis

Our analysis content is constantly being updated, so if you would like to know more, please contact our technical engineers.

Creative Proteomics provides detailed microbial genome de novo sequencing and analysis services, please contact us for more information. We deserve your trust.