Relying on advanced sequencing systems and cutting-edge technologies, Creative Proteomics provides global customers with whole genome bisulfite sequencing (WGBS) services to achieve genome-wide methylation analysis at the single C base level. To help you carry out research on basic mechanisms such as cell differentiation and tissue development, as well as animal and plant breeding, human health and disease treatment.
DNA methylation is a common epigenetic modification and a common and important mechanism in the regulation of gene expression. It is involved in many important life activities such as cell differentiation and proliferation, biological aging, and tumorigenesis. It plays an important role in the regulation of genomic imprinting, X chromosome inactivation, transposon inhibition, maintenance of epigenetic "memory" and tumorigenesis, and is one of the current research hotspots.
DNA methylation is an important epigenetic mark information. Obtaining the methylation level data of all C sites on a genome-wide scale has important implications for spatiotemporally specific studies of epigenetics. Whole genome bisulfite sequencing (WGBS), using bisulfite to treat genomic DNA to convert unmethylated cytosine (C) into uracil (U), and then perform whole genome sequencing on the treated DNA resequencing. Finally, it is mapped with the reference genome to achieve single-base resolution and high-precision methylation level analysis from the genome level.
For whole genome bisulfite sequencing (WGBS) of experimental samples, we followed the basic workflow shown below. At the same time, Creative Proteomics has developed a variety of new sample preparation technologies, and we will adjust the experimental methods and optimize the experimental conditions according to the different properties of the samples.
Type | Analyze content | |
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Data Analysis | Data quality control Data output statistics Mapping with reference genome Distribution of reads on gene functional elements |
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Standard bioinformatics analysis | Sequencing depth statistics
Genome-wide DNA methylation profiling
Differentially methylated promoter (DMP) analysis
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Advanced bioinformatics analysis | Methylation data SNP detection Tumor purity analysis Identification of methylated transcription factors Association analysis with other sequencing data |
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Personalized bioinformatics analysis | Discuss with our technical engineers to provide you with your own solution. |
Creative Proteomics provides detailed whole genome bisulfite sequencing (WGBS) and analysis services, please contact us for more information. We deserve your trust.
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