Whole Genome Bisulfite Sequencing (WGBS)
Relying on advanced sequencing systems and cutting-edge technologies, Creative Proteomics provides global customers with whole genome bisulfite sequencing (WGBS) services to achieve genome-wide methylation analysis at the single C base level. To help you carry out research on basic mechanisms such as cell differentiation and tissue development, as well as animal and plant breeding, human health and disease treatment.
Overview
DNA methylation is a common epigenetic modification and a common and important mechanism in the regulation of gene expression. It is involved in many important life activities such as cell differentiation and proliferation, biological aging, and tumorigenesis. It plays an important role in the regulation of genomic imprinting, X chromosome inactivation, transposon inhibition, maintenance of epigenetic "memory" and tumorigenesis, and is one of the current research hotspots.
DNA methylation is an important epigenetic mark information. Obtaining the methylation level data of all C sites on a genome-wide scale has important implications for spatiotemporally specific studies of epigenetics. Whole genome bisulfite sequencing (WGBS), using bisulfite to treat genomic DNA to convert unmethylated cytosine (C) into uracil (U), and then perform whole genome sequencing on the treated DNA resequencing. Finally, it is mapped with the reference genome to achieve single-base resolution and high-precision methylation level analysis from the genome level.
Technical advantages of whole genome bisulfite sequencing (WGBS):
- High sequencing accuracy: accurately realize single C base methylation status analysis.
- Wide detection range: with the help of high-throughput sequencing platform, the methylated regions in the whole genome can be studied.
- High reliability: directly sequence and quantify methylated fragments without cross-reaction and background noise.
- Cost-effective: using antibodies to enrich methylated regions for resequencing, the cost is low.
Advantages of Our Whole Genome Bisulfite Sequencing (WGBS) Services
- Reliable library construction quality: With years of technical experience, it can flexibly handle samples and library construction.
- Perfect quality control process: A series of quality control analysis will be carried out to ensure the accuracy of data.
- Rich basic analysis content: Comprehensive analysis of WGBS data from multiple perspectives.
- Personalized bioinformatics analysis services: mature bioinformatics analysis teams are familiar with various analysis algorithms and software; fully interact with customers to provide more personalized analysis ideas and analysis solutions.
Service Workflow
For whole genome bisulfite sequencing (WGBS) of experimental samples, we followed the basic workflow shown below. At the same time, Creative Proteomics has developed a variety of new sample preparation technologies, and we will adjust the experimental methods and optimize the experimental conditions according to the different properties of the samples.
Sample Requirements
- Tissue samples > 500 mg
- DNA: total amount >10 μg, concentration > 50 ng/μL, OD260/280 = 1.8~2.0
Bioinformatics Analysis
| Type |
Analyze content |
| Data Analysis |
Data quality control
Data output statistics
Mapping with reference genome
Distribution of reads on gene functional elements |
| Standard bioinformatics analysis |
Sequencing depth statistics
Genome coverage statistics
Mean methylation level calculation (C/CG/CHG/CHH)
Genome-wide methylation level distribution trend analysis
- Statistics of methylation levels of each chromosome
- Statistics of methylation levels in different gene regions
- Statistics of methylation levels of different gene elements
- Methylation motif sequence characterization analysis
Genome-wide DNA methylation profiling
Differential methylation site (DMS) analysis
Differentially methylated region (DMR) analysis
- DMR identification results statistics
- DMR structure annotation
- DMR gene GO and KEGG enrichment analysis
Differentially methylated promoter (DMP) analysis
- Differentially methylated promoter (DMP) statistics
- DMP cluster analysis
- DMP gene GO and KEGG enrichment analysis
|
| Advanced bioinformatics analysis |
Methylation data SNP detection
Tumor purity analysis
Identification of methylated transcription factors
Association analysis with other sequencing data |
| Personalized bioinformatics analysis |
Discuss with our technical engineers to provide you with your own solution. |
Creative Proteomics provides detailed whole genome bisulfite sequencing (WGBS) and analysis services, please contact us for more information. We deserve your trust.
Related Services
For Research Use Only.
