Creative Proteomics provides comprehensive analysis services of transcriptomics and proteomics to mine the correlation and mutual verification of transcriptome and proteome levels; screen key genes / proteins / metabolic pathways from massive data to provide powerful insights into the mechanism of complex life activities evidence. Then analyze the development of biological system processes, explain the complexity and integrity of biological processes.
Why Do Integrative Analysis of Transcriptomics and Proteomics?
Both transcriptomics and proteomics are important tools to obtain gene expression profiles. The transcriptome is an intermediate state of gene expression, reflecting mechanisms such as transcriptional regulation and post-transcriptional regulation; while proteins are the direct function executors of organisms, so the study of their expression levels has irreplaceable advantages. The differential characteristics of the samples at the gene expression level and the protein level were obtained through the full transcriptome and full proteome data. In order to reveal the regulatory influence mechanism between gene expression and protein, the association analysis of the two can be carried out based on "genes or proteins involved in the same biological process have the same or similar change rules". mRNA-protein associations are explored through integrated systematic analysis to explain biological questions holistically.
Technical Advantages
- Realize the analysis of expression levels at different levels and construct an expression "panorama".
- Multi-omics cross-validation: dig deep into the regulatory mechanism.
- Validation of gene mutation information at the protein level.
- Realize the transformation from genomics to protegenomics.
Our Integrative Analysis Services of Transcriptomics and Proteomics
Creative Proteomics provides you with one-stop transcriptome sequencing services and proteome detection services. In addition, we offer integrative analysis services of transcriptomics and proteomics to seamlessly connect your experiments upstream and downstream.
Bioinformatics Analysis
In order to obtain a collection of associated genes and proteins that have an impact on sample grouping, and analyze their associated characteristics, we mainly conduct 4-part analysis based on the expression abundance and functional information of genes and proteins: transcriptome-proteome association quantity statistics (and expression correlation analysis, expression cluster analysis), four-quadrant map analysis of differential genes and proteins, nine-quadrant map analysis of gene expression changes in the transcriptome and proteome, and GO/KEGG association analysis of differential genes and differential proteins.
Why Choose Us?
- Excellent bioinformatics analysis: Our bioinformatics center brings together many high-level information analysis talents from home and abroad, and has many years of practical experience in the field of bioinformatics research and development.
- Rich data analysis content: standard analysis, advanced analysis, customized analysis.
- In addition to excellent bioinformatics analysis, we also have an advanced experimental platform that supports sequencing and metabolome / proteome detection, etc., and we have many successful project experiences.
- Cost-effective, short project cycle.
Case Study
Integration of Transcriptome and Proteome Analysis Reveals the Mechanism of Freezing Tolerance in Winter Rapeseed
Journal: Plant Growth Regulation
Impact factor: 3.2418
Published: 2021
Backgrounds
Freezing stress are eventful for overwinter survival rate of winter rapeseed. However, the molecular mechanism of freezing tolerance formation is still very backward in winter rapeseed. In this study, using a pair of freezing-sensitive and freezing-resistant cultivars NQF24 and NTS57, the exhaustive effects of freezing stress on freezing tolerance formation were evaluated by analyzing leaf at the levels of transcriptome, proteome, physiology and ultrastructure.
Materials & Methods
Freezing-sensitive cultivars: NQF24
Freezing-resistant cultivars: NTS57
Transcriptome, proteome, physiology and ultrastructure
| Transcriptome sequencing | Proteome testing | Integrative analysis of transcriptimics + proteomics |
|---|---|---|
NQF24 leaf under freezing stress: 8497 differentially expressed genes (DEGs); NTS57 leaf: 7358 DEGs |
NQF24 leaf under freezing stress: 418 differentially abundant proteins (DAPs); NTS57 leaf: 573 DAPs |
Function enrichment analysis showed that most of the enriched DEGs and DAPs were associated with plant hormones signal transduction, fatty acid metabolism, ribosome, plant-pathogen interaction and secondary metabolites biosynthesis. |
Conclusions
After freezing treatment, rapeseed cultivar more tolerant to freezing stress would fabricate stronger signals (Ca2+, ROS, ABA, Auxin, BR, Ethylene and JA), metabolic pathways (protein biosynthesis, plant-pathogen interaction and secondary metabolites synthesis) and ROS scavenging capacity, more osmolytes (soluble sugar and protein) and lower level of lipid peroxidation in leaves, as well as more stable ultrastructure.
Fig.1 Comparison of transcripts and proteins abundance in developing winter rapeseed leaves. Venn diagram of identified DEGs. (A) and DAPs (B) in leaves of both cultivars, respectively; (C), Venn diagram of shared DEGs (C) and DAPs (D) in leaves of both cultivars, respectively. (Wei J, et al., 2021)
Creative Proteomics relies on advanced platforms and cutting-edge technologies to provide omics services to customers around the world, and provide accurate and detailed analysis reports. In addition, we also provide multi-omics integrated analysis services. You can discuss with our technical engineers to customize solutions or build new methods to meet your specific needs. If you have any questions, you can tell us through the inquiry form, and our technicians will communicate with you. Please don't hesitate to contact us, Creative Proteomics can be trusted.
Reference
- Wei J, Zheng G, Dong X, et al. Integration of Transcriptome and Proteome Analysis Reveals the Mechanism of Freezing Tolerance in Winter Rapeseed[J]. Plant Growth Regulation, 2021, 96(1):103-118.