With advanced sequencing systems and cutting-edge technologies, Creative Proteomics provides metatranscriptomic sequencing and analysis services to clients worldwide, helping you to accurately quantify the fine composition of active species and their corresponding functional expression levels across the entire bacteriophage, thereby targeting key biomarkers in the bacteriophage and elucidating their biological significance.
The metatranscriptome is a collection of RNAs (transcripts) of all microorganisms in a specific period of time and in a specific environment. Through metatranscriptomics sequencing, information on the transcriptomes of culturable and non-culturable microorganisms in the environment can be directly obtained to study the composition, active transcripts, and active functions of active species in microbial communities in a specific habitat at a specific time and space. It is also possible to compare differentially expressed genes and differential functional pathways in different environments, reveal the adaptation mechanisms of microorganisms under different environmental stresses, and explore the mechanisms of interaction between the environment and microorganisms.
For metatranscriptomics sequencing and analysis, we followed the basic workflow shown below. At the same time, Creative Proteomics has developed a variety of new sample preparation technologies, and we will adjust the experimental methods and optimize the experimental conditions according to the different properties of the samples.
Our analysis content is constantly being updated, so if you would like to know more, please contact our technical engineers.
Analysis type | Analysis content | |
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Standard bioinformatics analysis | Basic analysis | Sequence pre-processing and quality control mRNA sequence assembly and splicing |
Functional annotation and expression profiling | Functional annotation (KEGG / GO / EggNOG / CAZy) Expression distribution display Expression variance analysis (comparative analysis / clustering analysis) Shared / unique KEGG metabolic pathway analysis |
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Species annotation and composition profiling | Species annotation / difference analysis / clustering analysis Visualization display (Megan / GraPhlAn / Krona) Sparse curve / accumulation curve / rank curve analysis Species interaction network analysis SourceTracker analysis |
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Database specific annotation analysis | Secretion protein / T3SS prediction analysis Database annotation analysis (PHIVFDB / MvirDB / ARDB / CARD) |
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Advanced bioinformatics analysis | Alpha and beta diversity analysis | Alpha diversity analysis PCA / PCoA / NMDS / UPGMA analysis Shared function / species taxon analysis (Venn diagram) Function-species congruence analysis |
Key biomarker screening | LEfSe analysis RDA / CAP / PLS-DA analysis Adonis / PERMANOVA / ANOSIM analysis Random forest analysis |
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Personalised bioinformatics analysis | Please contact our technical engineers to discuss and evaluate. |
Creative Proteomics provides detailed metatranscriptomics sequencing and analysis services, please contact us for more information. We deserve your trust.
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Creative Proteomics, your unparalleled center of excellence in sequencing, mass spectrometry, and bioinformatics analysis platforms, is elevating the landscape of research. Our seamless integration of these cutting-edge capabilities ensures that clients receive extraordinary multi-omics joint analysis services.