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Metatranscriptomics Sequencing

With advanced sequencing systems and cutting-edge technologies, Creative Proteomics provides metatranscriptomic sequencing and analysis services to clients worldwide, helping you to accurately quantify the fine composition of active species and their corresponding functional expression levels across the entire bacteriophage, thereby targeting key biomarkers in the bacteriophage and elucidating their biological significance.

Overview

Metatranscriptomics Sequencing

The metatranscriptome is a collection of RNAs (transcripts) of all microorganisms in a specific period of time and in a specific environment. Through metatranscriptomics sequencing, information on the transcriptomes of culturable and non-culturable microorganisms in the environment can be directly obtained to study the composition, active transcripts, and active functions of active species in microbial communities in a specific habitat at a specific time and space. It is also possible to compare differentially expressed genes and differential functional pathways in different environments, reveal the adaptation mechanisms of microorganisms under different environmental stresses, and explore the mechanisms of interaction between the environment and microorganisms.

Technical advantages of metatranscriptomics sequencing

  • The technique reveals the true extent of microbial diversity.
  • Enables functional activity-based studies.
  • Cost-effective and time-efficient.
  • Wide range of applications, including basic research, human microecology, environment, industry, agriculture, etc.

Applications of metatranscriptomics sequencing

  • Ecosystem function studies.
  • Research on microorganisms in special environments.
  • Microbial interactions research. It helps to characterize functionally active bacterial and community metabolic interactions in microbial ecology studies.
  • It can contribute to clinical studies by identifying immune responses and triggering autoimmune processes.

Advantages of Our Metatranscriptomics Sequencing Services

  • Cutting-edge technology and multiple sequencing platforms to choose from flexibly.
  • Rich project experience.
  • High level analysis team, strong analysis capability, comprehensive analysis content.

Service Workflow

For metatranscriptomics sequencing and analysis, we followed the basic workflow shown below. At the same time, Creative Proteomics has developed a variety of new sample preparation technologies, and we will adjust the experimental methods and optimize the experimental conditions according to the different properties of the samples.

Service Workflow of Metatranscriptomics Sequencing -Creative Proteomics

Sample Requirements

  • RNA ≥ 6 μg, concentration ≥ 65 ng/μL, RIN ≥ 7.0, 28S/18S ≥ 1.0.

Bioinformatics Analysis

Our analysis content is constantly being updated, so if you would like to know more, please contact our technical engineers.

Analysis type Analysis content
Standard bioinformatics analysis Basic analysis Sequence pre-processing and quality control
mRNA sequence assembly and splicing
Functional annotation and expression profiling Functional annotation (KEGG / GO / EggNOG / CAZy)
Expression distribution display
Expression variance analysis (comparative analysis / clustering analysis)
Shared / unique KEGG metabolic pathway analysis
Species annotation and composition profiling Species annotation / difference analysis / clustering analysis
Visualization display (Megan / GraPhlAn / Krona)
Sparse curve / accumulation curve / rank curve analysis
Species interaction network analysis
SourceTracker analysis
Database specific annotation analysis Secretion protein / T3SS prediction analysis
Database annotation analysis (PHIVFDB / MvirDB / ARDB / CARD)
Advanced bioinformatics analysis Alpha and beta diversity analysis Alpha diversity analysis
PCA / PCoA / NMDS / UPGMA analysis
Shared function / species taxon analysis (Venn diagram)
Function-species congruence analysis
Key biomarker screening LEfSe analysis
RDA / CAP / PLS-DA analysis
Adonis / PERMANOVA / ANOSIM analysis
Random forest analysis
Personalised bioinformatics analysis Please contact our technical engineers to discuss and evaluate.

Creative Proteomics provides detailed metatranscriptomics sequencing and analysis services, please contact us for more information. We deserve your trust.

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Creative Proteomics, your unparalleled center of excellence in sequencing, mass spectrometry, and bioinformatics analysis platforms, is elevating the landscape of research. Our seamless integration of these cutting-edge capabilities ensures that clients receive extraordinary multi-omics joint analysis services.

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