Creative Proteomics provides transcriptome sequencing services based on high-throughput sequencing platforms, combined with lipidomics analysis services based on advanced tandem mass spectrometers and analysis software, and provides customized analysis solutions and fast / accurate analysis services. Creative Proteomics can provide you with one-stop transcriptomics and lipidomics integrated analysis services from experimental design, sample detection, data analysis, etc., to meet a variety of detection needs.
Why Do Integrative Analysis of Transcriptomics and Lipidomics?
Integrating transcriptomics and lipidomics data for integrated analysis can more systematically and comprehensively analyze the function and regulatory mechanism of biological lipid molecules, and screen out key lipid metabolism pathways and related genes, as well as lipid metabolites for further in-depth research.
Our Integrative Analysis Services of Transcriptomics and Lipidomics
Creative Proteomics provides you with one-stop transcriptome sequencing services and lipidome detection services. In addition, we offer integrative analysis services of transcriptomics and lipidomics to seamlessly connect your experiments upstream and downstream. To mine the correlation and mutual verification of transcriptome and lipidome levels; screen key genes / lipids / metabolic pathways from massive data to provide powerful insights into the mechanism of complex life activities evidence. Then analyze the development of biological system processes, explain the complexity and integrity of biological processes.
Bioinformatics Analysis
To obtain the set of relevant genes and lipids that have an impact on the sample grouping and to analyze their association characteristics, we performed the following analyses mainly based on the expression abundance and function of differential genes and differential lipids: pathway integration analysis of transcriptomics - lipidomics, multivariate statistical integration analysis of transcriptomics - lipidomics, WGCNA analysis, regulatory relationship analysis and correlation analysis of transcriptomics and lipidomics.
Why Choose Us?
- Excellent bioinformatics analysis - our bioinformatics center brings together many high-level information analysis talents from home and abroad, and has many years of practical experience in the field of bioinformatics research and development.
- Rich data analysis content - standard analysis, advanced analysis, customized analysis.
- In addition to excellent bioinformatics analysis, we also have an advanced experimental platform that supports sequencing and metabolome / proteome / lipidome detection, etc., and we have many successful project experiences.
- Cost-effective, short project cycle.
Case Study
Integrated lipidomic and transcriptomic analysis reveals triacylglycerol accumulation in castor bean seedlings under heat stress
Journal: Industrial Crops & Products
Impact factor: 6.449
Published: 2022
Backgrounds
Heat stress is one of the most challenging environmental factors for plants, which have evolved various mechanisms to cope with it. However, the role of lipid remodeling caused by heat stress is not yet fully understood.
Materials & Methods
Castor bean (Ricinus communis) var. ZB306
Transcriptome sequencing, lipid analysis, LC-MS, subcellular localization, qRT-PCR
| Transcriptomic analysis | Lipidomic analysis | Integrative analysis of lipidomic + transcriptomic |
|---|---|---|
| Transcriptomic analysis indicated that polyunsaturated fatty acids (FAs) used for TAGs are not derived from de novo synthesis, but likely from the lipids remodeling via lipase activity, and are subsequently esterified into TAGs via diacylglycerol acyltransferase (DGAT). | We detected 297 lipid compounds in castor bean seedlings, of which 54 displayed altered abundances under heat stress. In particular, polyunsaturated triacylglycerols (TAGs) and diacylglycerols (DAGs) significantly increased, while polyunsaturated monogalactosyldiacylglycerols (MGDGs) markedly decreased. When heat stress was subsequently relieved, these lipid molecules recovered to their normal levels. We found that heat-induced TAGs primarily accumulate in the cytosol. | Heat-induced changes in transcript levels coordinate changes in lipid metabolism. |
Conclusions
TAGs may act as intermediates in lipid turnover and provide one mechanism by which plants respond to heat stress, broadening our understanding of how lipid remodeling functions in plant adaptation to heat stress.
Fig.1 Molecular basis of TAG accumulation under heat stress. (Zhang Yanyu, et al., 2022)
Creative Proteomics relies on advanced platforms and cutting-edge technologies to provide omics services to customers around the world, and provide accurate and detailed analysis reports. In addition, we also provide multi-omics integrated analysis services. You can discuss with our technical engineers to customize solutions or build new methods to meet your specific needs. If you have any questions, you can tell us through the inquiry form, and our technicians will communicate with you. Please don't hesitate to contact us, Creative Proteomics can be trusted.
Reference
- Zhang Yanyu, Li Yelan, Han Bing, Liu Aizhong, Xu Wei. Integrated lipidomic and transcriptomic analysis reveals triacylglycerol accumulation in castor bean seedlings under heat stress[J]. Industrial Crops & Products,2022,180.