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Reduced Representation Bisulfite Sequencing (RRBS)

Relying on advanced sequencing systems and cutting-edge technologies, Creative Proteomics provides reduced representative bisulfite sequencing (RRBS) services to global customers to help you comprehensively study embryonic development, aging mechanisms, and epigenetic mechanisms of disease development, and screen disease-related epigenetic marker sites, etc.


Reduced Representation Bisulfite Sequencing (RRBS)

Reduced Representation Bisulfite Sequencing (RRBS) is an accurate, rapid, efficient and economical technology for DNA methylation research. Firstly, the fragments of CCGG sites on the genomic DNA are enriched by restriction enzyme digestion, and then by Bisulfite treatment and high-throughput sequencing, single-base resolution methylation sequencing in the CpG-enriched regions of the genome can be performed. This technology significantly improves the sequencing depth of CpG regions, and high-precision resolution can be obtained in CpG islands, promoter regions, and enhancer element regions. Therefore, we can study the methylation status of the covered regions in a targeted manner, making large-scale sample studies possible. It is widely used in large sample single base resolution methylation differential analysis and disease molecular marker development.

Technical advantages of Reduced Representation Bisulfite Sequencing (RRBS):

  • High precision: single base resolution; suitable for differential analysis between multiple samples.
  • Wide detection range: It covers more than 5 million CpG loci on a genome-wide scale.
  • Cost-effective: sequencing regions are more targeted and data utilization is higher.

Advantages of Our Reduced Representation Bisulfite Sequencing (RRBS) Services

  • Flexible experimental platform: Creative Proteomics mainly uses mainstream sequencing platforms, and can also flexibly utilize the advantages of different experimental platforms according to experimental purposes and requirements.
  • Cost-effective: We provide you with the most comprehensive services at the most favorable price to help you effectively save experimental costs.
  • With scientific scheme design, strict quality control management, professional analysis team and rich project experience, we promise to give you a high-quality project service.

Service Workflow

For reduced representation bisulfite sequencing (RRBS) of experimental samples, we followed the basic workflow shown below. At the same time, Creative Proteomics has developed a variety of new sample preparation technologies, and we will adjust the experimental methods and optimize the experimental conditions according to the different properties of the samples.

Service Workflow of Reduced Representation Bisulfite Sequencing (RRBS) -Creative Proteomics

Sample Requirements

  • Tissue samples > 500 mg
  • Cells ≥ 1x107
  • Genomic DNA ≥ 4 μg, concentration > 50 ng/μl, OD260/280 = 1.8-2.0, no RNA and protein contamination.
  • FFPE DNA samples ≥ 5 μg, OD260/280 = 1.8-2.0. There is no obvious degradation of DNA and protein contamination, and gel electrophoresis is required to detect gel images.

Bioinformatics Analysis

Type Analyze content
Advanced bioinformatics analysis

Alignment with reference genome

  • Read distribution analysis

Methylation site (MS) information analysis

  • Sites and number statistics of CG/CHG methylation
  • Relative quantitative analysis of methylation site levels
  • Distribution analysis of methylation sites on different gene functional elements

Genome-wide differential methylation site (DMS) analysis

  • DMS screening
  • DMS distribution analysis on different gene functional elements
  • DMS-related genes analysis between groups
  • Functional annotation of DMS-related genes
Personalized bioinformatics analysis Personalized analysis requires contacting our technical engineers for evaluation.

Creative Proteomics provides detailed reduced representation bisulfite sequencing (RRBS) and analysis services, please contact us for more information. We deserve your trust.

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