Relying on advanced sequencing systems and cutting-edge technologies, Creative Proteomics provides Small RNA sequencing and analysis services to customers around the world, aiming to discover, identify and quantify the genome-wide miRNA profiles of any species, and advance the research on miRNA functions.
Small RNA is an endogenous non-protein RNA with a length of 18-30 nt, which exists in almost all organisms, mainly including miRNA, piRNA and siRNA. It is an important regulator of life activities and participates in many life processes, such as cell proliferation, apoptosis, fat metabolism and cell differentiation. Therefore, it also plays an important role in the process of gene expression, biological ontogeny, metabolism and the occurrence of diseases.
For small RNA sequencing of experimental samples, we followed the basic workflow shown below. At the same time, Creative Proteomics has developed a variety of novel sample preparation techniques, and we will adjust the experimental methods and optimize the experimental conditions according to the different properties of the samples.
Sample Type | Requirements |
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Total RNA (complete and free of contamination) | ≥ 10 μg, concentration: ≥ 200 ng/μL; RIN ≥ 8.0, OD260/280 = 1.8-2.2, OD260/230 = 1.0-2.5. |
RNA from special samples, such as serum/plasma or co-immunoprecipitation |
≥ 50 ng, concentration ≥ 1 ng/μL. Impurities such as protein are required to be less contaminated and do not affect normal PAGE separation. |
Small RNA (< 200 nt) | ≥ 1μg, concentration ≥ 20ng/μL. |
Fresh animal and plant tissue | ≥ 1g |
Cell samples | ≥3x106 ~1x107. Lysed in lysis buffer and frozen for transport. |
Whole blood sample | ≥ 2 mL |
Do not freeze and thaw repeatedly during sample storage. Please use dry ice for shipping. |
Analysis type | Analyze content |
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Standard bioinformatics analysis | Raw data curation, filtering and quality assessment Statistical analysis of the length distribution of 18-30 nt small RNAs Alignment with reference genome Repeat sequence alignment Predict new miRNAs miRNA abundance differential analysis Annotation analysis
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Advanced bioinformatics analysis | Analysis of common / unique differential genes between groups miRNA family analysis Cluster analysis of differentially expressed miRNAs miRNA target gene prediction Target gene analysis (GO KEGG enrichment analysis) miRNA and mRNA interaction analysis |
Customized bioinformatics analysis | Customized analysis solutions according to the requirements of customers and the characteristics of the species themselves. |
If you are interested in small RNA sequencing, or you want to open up new research, you need to find a professional small RNA sequencing and analysis company for cooperation. Don't hesitate to get in touch with us today, Creative Proteomics can be trusted.
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Creative Proteomics, your unparalleled center of excellence in sequencing, mass spectrometry, and bioinformatics analysis platforms, is elevating the landscape of research. Our seamless integration of these cutting-edge capabilities ensures that clients receive extraordinary multi-omics joint analysis services.