Targeted Region Sequencing
Creative Proteomics uses advanced sequencing systems to provide targeted sequencing services covering any relevant gene, helping you obtain complete target sequences quickly and cost-effectively.
Overview
Targeted sequencing, also known as target region sequencing, is a technical means to capture and enrich genomic regions of interest and perform high-throughput sequencing. It can detect genetic variation sites in the target genome region, obtain the variation information of the specified target region, and analyze the target gene efficiently.
Technical advantages of targeted sequencing:
- Highly flexible: can identify low-frequency variants of alleles (as low as 1%)
- Micro library construction: the starting amount of library construction is as low as 10ng
- Ultra-high sequencing depth (500~1000X), more accurate data, and improved detection of rare variants
- Ultra-low detection limit: 0.1%
- Cost-effective: Short study period and lower cost, suitable for analysis of large sample size
Applications of targeted region sequencing include, but are not limited to:
- Detection of SNPs / InDels / CNVs / SVs
- Genotyping and classification of eukaryotic and/or prokaryotic cell lines
- Detection and quantification of rare variants and low-frequency alleles
- Linkage analysis of genetic diseases
- Discover and validate disease-related candidate genes or associated loci
- Discovery of biomarkers and therapeutic targets
- Design genetic tests, such as pharmacogenomic tests or oncology tests
Advantages of Our Targeted Region Sequencing Services
- Scientific solutions, sophisticated technology, advanced sequencing platform.
- Experienced scientific experts are here to support you.
- Cost-effective, can complete mixed detection of a large number of samples.
- For reliable downstream analysis, we offer comprehensive bioinformatics service options that seamlessly connect your upstream and downstream experiments.
Service Workflow
For targeted region sequencing of experimental samples, we followed the basic workflow shown below. At the same time, Creative Proteomics has developed a variety of new sample preparation technologies, and we will adjust the experimental methods and optimize the experimental conditions according to the different properties of the samples.
Sample Requirements
- DNA: amount ≥ 10 ng, concentration ≥ 1 ng/μL, OD260/280 = 1.8~2.0.
- Sample type: purified genomic DNA, tissue samples, blood, frozen cell pellets, PCR amplicons, bacterial colonies, formalin-fixed / paraffin-embedded (FFPE) sections, cotton swabs, etc.
Bioinformatics Analysis
| Type |
Analyze content |
| Data preprocessing |
Data quality control: remove connector contamination and low quality data
Align with reference sequences, and count sequencing depth and coverage
SNP/InDel detection, annotation and statistics
Somatic SNV/InDel detection, annotation and statistics for paired samples |
| Standard analysis |
Statistical and pathway enrichment analysis of high-frequency mutant genes (>2 pairs of samples)
Correlation analysis of MRT high-frequency mutation genes (>2 pairs of samples)
NovoDriver known driver gene screening
OncodriveCLUST driver gene prediction (>2 pairs of samples)
Mutation site distribution
Display of SNP/InDel mutation sites for frequently mutated genes
Display of SNP/InDel mutation sites for predicted driver genes
NovoDrug high-frequency mutation gene targeted drug prediction
NovoDR resistance mutation screening |
| Customized analysis |
You can discuss with our technical engineers and conduct customized bioinformatic analysis. |
Creative Proteomics has extensive experience in providing targeted sequencing services to clients around the world. We can provide one-stop solutions from project consulting and experimental design to sequencing and bioinformatics analysis. We are confident to provide our customers with the best service at the most competitive cost. For more service information, please feel free to contact us.
Related Services
For Research Use Only.
