Overview

Whole transcriptome is the sum of all transcripts, including mRNA and non-coding RNA (ncRNA), that can be transcribed in a specific species, tissue or cell type under a certain period or condition. The so-called whole transcriptome sequencing mainly detects mRNA and three ncRNAs (ie lncRNA, circRNA and miRNA). Through whole transcriptome sequencing, interaction analysis between various RNAs, co-expression analysis, and competitive endogenous RNA (ceRNA) network analysis can be achieved. CeRNA is one of the hottest topics in the field of transcriptional regulation, and the molecular mechanism of ceRNA can be systematically elucidated through whole transcriptome studies.

Applications of whole transcriptome sequencing and analysis:

• Growth and development regulation (animal metamorphosis development, plant flowering, fruit development, etc.)
• Environmental adaptation mechanisms (drought, salt tolerance, disease resistance, etc.)
• Immune interactions (bacterial, fungal, viral infection)
• Disease development research
• Drug target research

Advantages of Our Whole Transcriptome Sequencing and Analysis Services

• Complete and advanced platform.
• Rich library construction experience, excellent library construction quality and complex sample processing capabilities.
• According to the needs of customers, customized information analysis can be flexibly carried out.
• Featured advanced analysis: lncRNA regulatory mechanism prediction, co-expression analysis of lncRNA and mRNA, integrated analysis of lncRNA, mRNA and circRNA, etc.

Service Workflow

For whole-transcriptome sequencing of experimental samples, we followed the basic workflow shown below. At the same time, Creative Proteomics has developed a variety of novel sample preparation techniques, and we will adjust the experimental methods and optimize the experimental conditions according to the different properties of the samples.

Sample Requirements (Recommended)

• Animal and plant tissue: fresh weight ≥ 1.8 g (effective tissue cell weight)
• Fungus: wet weight ≥ 1.8 g (fungal entity, mycelium, spore powder, etc.)
• Animal, plant and yeast cells ≥ 6×10⁷
• Fresh blood (anticoagulation) ≥ 2.25 mL
• RNA: concentration ≥ 200 ng/µl, total amount ≥ 10 µg

Bioinformatics Analysis

If you are interested in whole transcriptome sequencing, or you want to open up new research, you need to find a professional whole transcriptome sequencing analysis company for cooperation. Don't hesitate to get in touch with us today, Creative Proteomics can be trusted.