Qualitative Proteomic Analysis
Relying on advanced sequencing systems and cutting-edge technologies, Creative Proteomics provides global customers with qualitative proteomic analysis services. We provide one-stop service for protein extraction, mass spectrometry detection and data analysis, to help you conduct refined proteome studies.
Overview
To understand proteins, we must first understand the properties of proteins, that is, qualitative analysis of the proteome. In addition to using protein analysis software to predict various parameters, the isoelectric point, molecular weight, primary structure and secondary structure are analyzed by instruments and equipment for verification and analysis. After comprehensive analysis of the protein, the function and mechanism of action can be further inferred.
Qualitative protein analysis usually refers to protein identification and sequence analysis using methods such as mass spectrometry.
Applications of qualitative proteomic analysis
- Protein spot identification
- Protein interaction identification
- Protein mixture identification
- Modified qualitative proteome
Qualitative Proteomic Analysis We Offer
According to the type of samples can be divided into:
- Protein full-spectrum analysis (i.e. shotgun analysis)
- Protein strip / mixture analysis
- Protein spot analysis
Advantages of Our Qualitative Proteomic Analysis Services
- One-stop service: We not only provide qualitative analysis of proteome, but also provide protein expression, antibody preparation and mutual experiments.
- Comprehensive project: A full set of analytical instruments, professional laboratory personnel, and accurate measurement results meet diverse experimental needs.
- Customized service: We also offer other technical services such as electroblotting and depyroglutamate cyclization.
Service Workflow
For qualitative proteomic analysis services, we followed the basic workflow shown below. At the same time, Creative Proteomics has developed a variety of new sample preparation technologies, and we will adjust the experimental methods and optimize the experimental conditions according to the different properties of the samples.
Service content
| Analysis project |
Illustrate |
| N-terminal sequencing |
N-terminal sequencing instrument |
| C-terminal sequencing |
MS analysis |
| Peptide sequence determination |
MS analysis |
| Protein full sequence determination |
MS analysis |
| Monoclonal antibody sequencing |
MS analysis |
| Protein molecular weight determination |
MS analysis / MALDI-TOF analysis |
| Protein purity identification |
HPLC analysis |
| Protein primary structure analysis |
MS analysis |
| Protein disulfide bond localization |
MS analysis |
| Protein isoelectric point analysis |
Flat plate isoelectric focusing |
| Circular dichroism analysis |
Circular dichroism instrument |
Sample Requirements
| Service type |
Sample requirements |
| Protein full-spectrum analysis (shotgun) |
- Protein solution: total protein > 10 μg, solution concentration > 0.5 μg/μL, buffer does not contain NP40, Triton, X-100 and other detergents.
|
| Protein strip / mixture analysis |
- SDS-PAGE bands: bands are clearly visible.
- Protein mixed solution: total protein > 10 μg, solution concentration > 0.5 μg/μL, buffer does not contain NP40, Triton, X-100 and other detergents.
|
| Protein spot identification |
- Two-dimensional gel electrophoresis spots: glue spots are clearly visible.
- SDS-PAGE bands: single protein purity > 90%, clearly visible bands.
- Protein solution: single protein purity > 90%; Total protein > 10 μg, solution concentration > 0.5 μg/μL.
|
Creative Proteomics provides detailed qualitative proteomic analysis services, please contact us for more information. We deserve your trust.
Related Services
For Research Use Only.
